Eppendorf BioPhotometer® D30

Image – BioPhotometer D30 front
Image – VIS Cuvette macro
Image – photometric measurement Application Note 279
Image – VIS Cuvette semi-micro
Image – µCuvette G1.0
Image – BioPhotometer D30 µCuvette
Image – UVette light paths_white
Image – BioPhotometer D30 workflow 02
Image – BioPhotometer D30 front
Image – VIS Cuvette macro
Image – photometric measurement Application Note 279
Image – VIS Cuvette semi-micro
Image – µCuvette G1.0
Image – BioPhotometer D30 µCuvette
Image – UVette light paths_white
Image – BioPhotometer D30 workflow 02
Eppendorf BioPhotometer® D30

UV-vis is your standard?
The Eppendorf BioPhotometer D30 is the third generation of Eppendorf's well-established line of BioPhotometers. The compact instrument clearly processes data, records at fixed wavelengths and makes evaluating results fast and simple. For specific methods, additional measurements are recorded and displayed as a graph, making impurities easier and quicker to identify. More information

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The Eppendorf BioPhotometer D30 is the 3rd generation of Eppendorf spectrophotometers to become an established standard in the life sciences. Routine pre-defined wavelengths are easily selectable, allowing for quick and simple programming. Users are guided through every step of the assay with short, simple on-screen instructions. The ultra-stable Xenon flash lamp doesn't require any warm up time and a measurement typically takes 5 seconds. Raw and processed data are clearly presented on the large color screen and can be exported directly from the instrument in a variety of common formats. Eppendorf disposable UVette® cuvettes enable measurements with as little as 50 µL of sample and the Eppendorf µCuvette® G1.0 reduces this further to 1.5 µL of sample.

Nucleic acid purification is a major application in molecular laboratories. Purity and homogeneity of the sample are important considerations for subsequent applications. In practice, nucleic acids are purified with the help of commercially available kits which allow for the separation of most cellular components. However, the presence of proteins or other organic components in the eluate may not be entirely eliminated.
When purifying nucleic acid without a kit, additional contamination risks are posed by the chemicals used in the process, e.g. phenol or ethanol from a phenol/chloroform extraction. To ensure minimum contamination of the sample, it is essential to verify its purity by photometric measurements.
The ratios of the absorbance values at the wavelengths 230 nm, 260 nm and 280 nm (260 nm/230 nm and 260 nm/280 nm), provide a clear picture of the purity of a nucleic acid sample. The following values represent a pure DNA sample:
A260/A230 ≥ 2.0
A260/A280 = 1.8 – 2.0

Believing in raw data?

In general, the purity of a DNA solution is easily defined using purity ratios. It is possible that reduced purity may be attributed to the fact that the sample is solubilized in water instead of in buffer. Since water and buffer have different properties, the absorbance of the sample being measured will be affected by this disparity.
For this reason, the BioPhotometer D30 is capable of recording a “Purity Scan” which displays the absorbance properties of a sample graphically, thus enabling fast and simple visual quality control.

In addition, all important raw data pertaining to the DNA sample are displayed in table format. The table optionally lists the purity ratios (A260/A230 and A260/A280), the absorbance values (raw data), as well as the background absorbance, e.g. at 320 nm.
Thus, the two display formats complement each other perfectly to allow for an evaluation of the quality of a DNA sample.

Eppendorf BioPhotometer® D30

Annoyed by uncomfortable handling?

Ergonomics is far beyond the »ergonomically designed chair«. Eppendorf started to optimize the laboratory devices regarding ergonomics already in the early 1970s.
In 2003, we started the PhysioCare Concept, focusing on ergonomic liquid handling devices like our pipettes.
Nowadays, the Eppendorf PhysioCare Concept is broaden up to include further laboratory products as a holistic solution to harmonize the workflow in your laboratory with your health and well-being, e.g. for the Eppendorf BioSpectrometer:
› Guided software for easy handling
› Clear indications at cuvette shaft for convenient handling
› Direct keys for convenient handling
› Small footprint to enable position in direct arm reach at your bench
› Standardized »user-interface concept« for intuitive handling of devices

Further information about the Eppendorf PhysioCare Concept:

Eppendorf BioPhotometer® D30

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Eppendorf BioPhotometer® D30


  • Absorption measurements for one or more fixed wavelengths
  • Scan for specific UV applications with automatically determined purity ratios
  • Preprogrammed applications for quick start and applications with evaluation via factor, standard or standard series
  • Integrated application and results memory
  • Guided software process to minimize errors
  • Compatible with standard, semi-micro and micro-volume measuring cells
  • Fixed wavelengths without moving parts for a long life time
  • Integrated self-test and calibration history for documentation
  • Data transfer via USB interface, Ethernet or via e-mail and direct printout of results
  • Data output as *.PNG screenshots, Microsoft® Excel®, or secure PDF files to a standard USB flash drive or via email


  • Nucleic acid quantification
  • Direct protein quantification (UV 280 nm)
  • Microvolume measurement via µCuvette G1.0 for highly concentrated samples
  • Bacteria growth measurement (OD 600)
  • Colorimetric assays for protein quantification, e.g., BCA, Bradford, Lowry
  • 340 nm: endpoint assays using NADPH or NAPH
  • 405 nm: endpoint assays using para-nitrophenol
  • 490 nm: cytotoxicity endpoint assay
  • Transmission measurement
Eppendorf BioPhotometer® D30
Video: Eppendorf BioPhotometer® D30 – Video – University of East London

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