Eppendorf BioSpectrometer® kinetic

Eppendorf BioSpectrometer® kinetic
Eppendorf BioSpectrometer® kinetic
Eppendorf BioSpectrometer® kinetic
Eppendorf BioSpectrometer® kinetic
Eppendorf BioSpectrometer® kinetic
Eppendorf BioSpectrometer® kinetic
Eppendorf BioSpectrometer® kinetic
Eppendorf BioSpectrometer® kinetic
Eppendorf BioSpectrometer® kinetic
Eppendorf BioSpectrometer® kinetic
Eppendorf BioSpectrometer® kinetic
Eppendorf BioSpectrometer® kinetic
Eppendorf BioSpectrometer® kinetic
Eppendorf BioSpectrometer® kinetic
Eppendorf BioSpectrometer® kinetic
Eppendorf BioSpectrometer® kinetic
Eppendorf BioSpectrometer® kinetic
Eppendorf BioSpectrometer® kinetic
Eppendorf BioSpectrometer® kinetic
Eppendorf BioSpectrometer® kinetic
. Eppendorf BioSpectrometer® kinetic

The Eppendorf BioSpectrometers are spectrophotometers that combine easy handling with highly reproducible analysis. A variety of cuvettes expands the options even further from microvolume to macrovolume measurements. More information

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Order Info

Product Information

The Eppendorf BioSpectrometer kinetic is an expansion of the Eppendorf BioSpectrometer basic and offers preprogrammed and freely programmable timed and temperature controlled assays. It features an integrated heated cuvette shaft. Its integrated Peltier element guarantees high-precision for determining enzyme and substrate kinetics as well as other temperature-sensitive applications. Guided software is included; minimal effort is required to set up new assays. Retrospective modification of the time window for regression analysis can be made directly on the device. The BioSpectrometer kinetic replaces the traditional large cumbersome setup required for temperature controlled timed assays.

Features

  • All Eppendorf BioSpectrometer basic options
  • Integrated temperature-controlled cuvette shaft (adjustment range +20 °C to +42 °C in 0.1 °C increments)
  • Integrated interval timer (minimum interval 5 s, maximum measuring time range 59 m 59 s)
  • Preprogrammed and freely programmable kinetic methods
  • Retrospective modification of the time window for regression analysis
  • Data output via JPEG screenshots, Microsoft Excel or secure PDF files to a standard USB flash drive or via email

Applications

  • All Eppendorf BioSpectrometer basic applications
  • Additional kinetic applications, and enzyme activity testings at different temperatures e.g.:
  • Hexokinase activity measurements (340 nm, 37 °C)
  • Determination of ATP concentration (340 nm, 37 °C)
  • Beta-galactosidase assays (420 nm, 30 °C)
 Eppendorf BioSpectrometer® kinetic
Absorption measuring range0 A – 3.0 A (260 nm)
BSA concentration range (UV 280 nm) µCuvette G1.0758 ng/µL - 45,450 ng/µL
±30 ng/µL at A = 0
±76 ng/µL at A = 1 (0.5 %)
BSA concentration range (UV 280 nm) UVette 10 mm75 ng/µL - 4,545 ng/µL
±3 ng/µL at A = 0
±7 ng/ µL at A = 1 (0.5 %)
BSA concentration range (UV 280 nm) UVette 2 mm379 ng/µL - 22,725 ng/µL
±15 ng/µL at A = 0
±38 ng/µL at A = 1 (0.5 %)
BSA concentration range (for 1 to 10 mm path length)76 ng/µL - 45,450 ng/µL
(The factor for BSA is 1,515)
Cuvette shaft12.5 mm × 12.5 mm, tempered 20 °C – 42 °C
Detector TypeCMOS photodiode array, 1024 pixel
Emission wavelength I fluorescence
Emission wavelength II fluorescence
Excitation wavelength fluorescence
Fluorescence measuring range
Light source absorptionXenon flash lamp
Light source fluorescence
Measuring principle absorptionSingle-beam absorption spectrophotometer with reference beam
Measuring principle fluorescence
Method memory>100 method programs
Operator guidance languageSpanish, Italian, French, English, German, Japanese
Power consumptionApprox. 30 W during operating step
Approx. 5 W with dimmed display and temperature control switched off
Random error absorption≤0.002 at A = 0
≤0.005 (0.5 %) at A = 1
Random error fluorescence
Random error wavelength±1 nm
Random temperature error±0.15 °C at 25 – 37 °C
Sample light path length8.5 mm
Scanningyes
Smallest step size1 nm
Spectral bandwidth≤4 nm
Systematic error absorption±1 % (A = 1)
Systematic error wavelength≤0.5 nm
Systematic temperature error±0.2 °C at 25 – 37 °C
Wavelength Selection Increment1 nm
Wavelength range200 nm – 830 nm
Wavelength range absorptionScan (nm): 200 – 830 at 1 nm increments
dsDNA concentration range (UV 260 nm) UVette 10 mm2.5 ng/µL – 150 ng/µL
dsDNA concentration range (UV 260 nm) UVette 2 mm12.5 ng/µL – 750 ng/µL
dsDNA concentration range (UV 260 nm) µCuvette G1.025 ng/µL – 1,500 ng/µL
dsDNA concentration range (for 1 to 10 mm path length)2.5 ng/µL – 1,500 ng/µL
Interfaces› USB master: for USB stick and thermal printer DPU-S445
› USB slave for connecting to a PC (All functions available without PC)
› Serial interface RS-232: for thermal printer DPU-414
› Ethernet interface RJ45: For connecting to a network printer or emailing data outputs directly off instrument
Power supply230 V, 50 – 60 Hz
Dimensions (W × D × H)295 × 400 × 150 mm / 11.6 × 15.7 × 6 in
Weight w/o accessories5.5 kg
Interval between two measuring points00:05 – 10:00 min:sec
Measuring time range00:05 – 59:59 min:sec
Adjustable temperature range20 – 42 °C (smallest step size 0.1 °C)

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