Mastercycler® X50

Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
Mastercycler® X50
. Mastercycler® X50

The new Mastercycler X50 is the elegant synthesis of speed, flexibility and PCR optimization for research PCR applications and standardization for routine PCR applications such as food testing etc. A new highly intuitive touch screen concept puts all of those benefits always at your fingertips. More information

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Product Information

The Mastercycler X50 family boasts ramp rates of up to 10°C/s and is the ideal cycler family for routine applications as well as advanced research in molecular biology.

The Mastercycler X50 family consist of units with a user-friendly touchscreen interface or eco units which are externally controlled. Each cycler with a touchscreen can be networked with nine additional eco units. If that is not enough throughput, you can network up fifty Mastercycler eco units to a computer or laptop running CycleManager X50 as a central controller. Whichever you choose, you can independently control and monitor every unit. You can also manage who has access to the system and designate a level of access with our intuitive user interface. Low noise levels, low power consumption, and the versatile flexlid® concept complete the product to be a powerful and convenient PCR cycler.

PCR Optimization of β-actin Gene with 2D-Gradient technique.


2D-Gradient allows you to optimize both the denaturation temperature (bottom to top) and the annealing temperature (left to right) during the same run. Higher denaturation temperatures can have the advantage of increased specificity while lower denaturation temperatures reduce stress on biomolecules and can lead to increased yield. Assays that struggle to work reliably at a 95° denaturation temperature could benefit significantly from the optimization of the denaturation temperature.

Mastercycler® X50

PCR should be faster. That gives you quicker results, throughout the day, and a faster transition to optimal temperatures. The Mastercycler X50 heats with 10 °C/s and cools with 5 °C/s. Make the speed of the Mastercycler X50 your research advantage.

Mastercycler® X50

There ia a large variety of cyclers available with different ramp rates published.
If you compare the corresponding run times by performing the same protocol using different cyclers, you see that ramp rates do not tell the full story about whether a cycler is fast or not. For more details, see Application Note 274.

Mastercycler® X50

Features

  • Heating rate: up to 10 °C/s
  • Wide selection of blocks from a fast silver block to 384 well block
  • Touch screen interface
  • Innovative 2D-Gradient for advanced PCR optimization
  • X50 with Touchscreen can control up to 9 other units (connected cyclers do not have a touch screen interface)
  • flexlid® concept: automatic height adjustment of the lid allows you to use all types of consumables
  • Small footprint
  • 2 year warranty

Applications

  • Fast PCR
  • PCR optimization
  • Standard PCR
  • Incubation
  • Cycle sequencing
 MC X50sMC X50aMC X50pMC X50hX50iX50lX50rX50t
Touchscreen Interface
Unit can control 9 other units in network
Unit can only be controlled
ThermoblockSilverAluminumAluminumAluminumSilverAluminumAluminumAluminum
Sample capacity96 × 0.2 mL PCR vessels, 1 PCR plate (96 wells)96 × 0.2 mL PCR vessels, 1 PCR plate (96 wells)1 PCR plate (96 wells)1 PCR plate (384 wells)96 × 0.2 mL PCR vessels, 1 PCR plate (96 wells)96 × 0.2 mL PCR vessels, 1 PCR plate (96 wells)1 PCR plate (96 wells)1 PCR plate (384 wells)
96-well plate
384-well plate
0.1/0.2 mL tubes
Heating technologySix peltier elementsSix peltier elementsSix peltier elementsSix peltier elementsSix peltier elementsSix peltier elementsSix peltier elementsSix peltier elements
2D-Gradient blockover 12 columns/over 8 rowsover 12 columns/over 8 rowsover 12 columns/over 8 rowsover 24 columns/over 16 rowsover 12 columns/over 8 rowsover 12 columns/over 8 rowsover 12 columns/over 8 rowsover 24 columns/over 16 rows
Temperature control modeFast, Intermediate, Standard, SafeFast, Intermediate, Standard, SafeFast, Intermediate, Standard, SafeFast, Intermediate, Standard, SafeFast, Intermediate, Standard, SafeFast, Intermediate, Standard, SafeFast, Intermediate, Standard, SafeFast, Intermediate, Standard, Safe
Temperature control range of the block4 – 99 °C4 – 99 °C4 – 99 °C4 – 99 °C4 – 99 °C4 – 99 °C4 – 99 °C4 – 99 °C
Gradient range1 – 30 °C1 – 30 °C1 – 30 °C1 – 30 °C1 – 30 °C1 – 30 °C1 – 30 °C1 – 30 °C
Gradient temperature range30 – 99 °C30 – 99 °C30 – 99 °C30 – 99 °C30 – 99 °C30 – 99 °C30 – 99 °C30 – 99 °C
Block homogeneity 20–72 °C≤±0.2 °C≤±0.2 °C≤±0.2 °C≤±0.2 °C≤±0.2 °C≤±0.2 °C≤±0.2 °C≤±0.2 °C
Block homogeneity 95 °C≤±0.3 °C≤±0.3 °C≤±0.3 °C≤±0.3 °C≤±0.3 °C≤±0.3 °C≤±0.3 °C≤±0.3 °C
Control accuracy±0.15 °C±0.15 °C±0.15 °C±0.15 °C±0.15 °C±0.15 °C±0.15 °C±0.15 °C
Heating rate10 °C/s5 °C/s5 °C/s5 °C/s10 °C/s5 °C/s5 °C/s5 °C/s
Cooling rate5 °C/s2.3 °C/s2.3 °C/s2.3 °C/s5 °C/s2.3 °C/s2.3 °C/s2.3 °C/s
Lid descentflexlid® technology with TSPflexlid® technology with TSPflexlid® technology with TSPflexlid® technology with TSPflexlid® technology with TSPflexlid® technology with TSPflexlid® technology with TSPflexlid® technology with TSP
High pressure lid
Lid temperature range37 – 110 °C37 – 110 °C37 – 110 °C37 – 110 °C37 – 110 °C37 – 110 °C37 – 110 °C37 – 110 °C
InterfacesEthernet, USBEthernet, USBEthernet, USBEthernet, USBEthernet, USBEthernet, USBEthernet, USBEthernet, USB
Power supply110 – 230 V, 50 – 60 Hz110 – 230 V, 50 – 60 Hz110 – 230 V, 50 – 60 Hz110 – 230 V, 50 – 60 Hz110 – 230 V, 50 – 60 Hz110 – 230 V, 50 – 60 Hz110 – 230 V, 50 – 60 Hz110 – 230 V, 50 – 60 Hz
Max. power consumption850 W850 W850 W850 W850 W850 W850 W850 W
Total weight11.5 kg11.5 kg11.5 kg11.5 kg10.7 kg10.7 kg10.7 kg10.7 kg
Dimensions (W × D × H)27,5 × 43 × 33 cm27,5 × 43 × 33 cm27,5 × 43 × 33 cm27,5 × 43 × 33 cm27,5 × 43 × 33 cm27,5 × 43 × 33 cm27,5 × 43 × 33 cm27,5 × 43 × 33 cm

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